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Generating CRISPR/Cas9-Derived Mutant Mice by Zygote Cytoplasmic Injection Using an Automatic Microinjector

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) assisted generation of mutant animals has become the method of choice for the elucidation of gene function in development and disease due to the shortened timelines for generation of a desired mutant, the ease...

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Detalhes bibliográficos
Publicado no:Methods Protoc
Main Authors: Doe, Brendan, Brown, Ellen, Boroviak, Katharina
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6526459/
https://ncbi.nlm.nih.gov/pubmed/31164552
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/mps1010005
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