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Switching the activity of Cas12a using guide RNA strand displacement circuits
The CRISPR effector protein Cas12a has been used for a wide variety of applications such as in vivo gene editing and regulation or in vitro DNA sensing. Here, we add programmability to Cas12a-based DNA processing by combining it with strand displacement-based reaction circuits. We first establish a...
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| Udgivet i: | Nat Commun |
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| Main Authors: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Nature Publishing Group UK
2019
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6504869/ https://ncbi.nlm.nih.gov/pubmed/31064995 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-019-09953-w |
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