Enhancement of target specificity of CRISPR–Cas12a by using a chimeric DNA–RNA guide

The CRISPR–Cas9 system is widely used for target-specific genome engineering. CRISPR–Cas12a (Cpf1) is one of the CRISPR effectors that controls target genes by recognizing thymine-rich protospacer adjacent motif (PAM) sequences. Cas12a has a higher sensitivity to mismatches in the guide RNA than doe...

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Détails bibliographiques
Publié dans:Nucleic Acids Res
Auteurs principaux: Kim, Hanseop, Lee, Wi-jae, Oh, Yeounsun, Kang, Seung-Hun, Hur, Junho K, Lee, Hyomin, Song, WooJeung, Lim, Kyung-Seob, Park, Young-Ho, Song, Bong-Seok, Jin, Yeung Bae, Jun, Bong-Hyun, Jung, Cheulhee, Lee, Dong-Seok, Kim, Sun-Uk, Lee, Seung Hwan
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 2020
Sujets:
Nucleic Acid Enzymes
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7470973/
https://ncbi.nlm.nih.gov/pubmed/32687187
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa605
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