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Synthesis and Characterization of Site-Specific O(6)-Alkylguanine DNA-alkyl Transferase-Oligonucleotide Crosslinks

O(6)-Alkylguanine DNA-alkyltransferase (AGT), a DNA repair protein, can form crosslinks with DNA. The AGT-DNA crosslinks are known to be mutagenic when AGT is heterologously expressed in Escherichia coli, as well as in mammalian cells. To understand the biological consequences, reliable access to AG...

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Détails bibliographiques
Publié dans:Curr Protoc Nucleic Acid Chem
Auteurs principaux: Ghodke, Pratibha P., Albertolle, Matthew E., Johnson, Kevin M., Guengerich, F. Peter
Format: Artigo
Langue:Inglês
Publié: 2019
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6504252/
https://ncbi.nlm.nih.gov/pubmed/30657645
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpnc.74
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