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In vitro evolution of enhanced RNA replicons for immunotherapy

Self-replicating (replicon) RNA is a promising new platform for gene therapy, but applications are still limited by short persistence of expression in most cell types and low levels of transgene expression in vivo. To address these shortcomings, we developed an in vitro evolution strategy and identi...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Sci Rep
Hauptverfasser: Li, Yingzhong, Teague, Brian, Zhang, Yuan, Su, Zhijun, Porter, Ely, Dobosh, Brian, Wagner, Tyler, Irvine, Darrell J., Weiss, Ron
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group UK 2019
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6502795/
https://ncbi.nlm.nih.gov/pubmed/31061426
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-43422-0
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