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Thermodynamics of nanobody binding to lactose permease

Camelid nanobodies (Nbs) raised against the outward-facing conformer of a double-Trp mutant of the lactose permease of Escherichia coli (LacY) stabilize the permease in outward-facing conformations. Isothermal titration calorimetry is applied herein to dissect the binding thermodynamics of two Nbs,...

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Bibliografske podrobnosti
izdano v:Biochemistry
Main Authors: Hariharan, Parameswaran, Andersson, Magnus, Jiang, Xiaoxu, Pardon, Els, Steyaert, Jan, Kaback, H. Ronald, Guan, Lan
Format: Artigo
Jezik:Inglês
Izdano: 2016
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6478161/
https://ncbi.nlm.nih.gov/pubmed/27686537
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.6b00826
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