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Transient conformers of LacY are trapped by nanobodies

The lactose permease of Escherichia coli (LacY), a highly dynamic membrane protein, catalyzes symport of a galactopyranoside and an H(+) by using an alternating access mechanism, and the transport cycle involves multiple conformational states. Single-domain camelid nanobodies (Nbs) developed against...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Smirnova, Irina, Kasho, Vladimir, Jiang, Xiaoxu, Pardon, Els, Steyaert, Jan, Kaback, H. Ronald
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4653160/
https://ncbi.nlm.nih.gov/pubmed/26512108
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1519485112
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