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Thermodynamics of nanobody binding to lactose permease
Camelid nanobodies (Nbs) raised against the outward-facing conformer of a double-Trp mutant of the lactose permease of Escherichia coli (LacY) stabilize the permease in outward-facing conformations. Isothermal titration calorimetry is applied herein to dissect the binding thermodynamics of two Nbs,...
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| Publicado en: | Biochemistry |
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| Autores principales: | , , , , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
2016
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6478161/ https://ncbi.nlm.nih.gov/pubmed/27686537 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.6b00826 |
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