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Direct observation of individual tubulin dimers binding to growing microtubules

The biochemical basis of microtubule growth has remained elusive for over 30 years despite being fundamental for both cell division and associated chemotherapy strategies. Here, we combine interferometric scattering microscopy with recombinant tubulin to monitor individual tubulins binding to and di...

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Bibliographic Details
Published in:Proc Natl Acad Sci U S A
Main Authors: Mickolajczyk, Keith J., Geyer, Elisabeth A., Kim, Tae, Rice, Luke M., Hancock, William O.
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 2019
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6462098/
https://ncbi.nlm.nih.gov/pubmed/30804205
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1815823116
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