Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression

Spatial/temporal control of Cas9 guide RNA expression could considerably expand the utility of CRISPR-based technologies. Current approaches based on tRNA processing offer a promising strategy but suffer from high background. Here, to address this limitation, we present a screening platform which al...

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Udgivet i:Nat Commun
Main Authors: Knapp, David J. H. F., Michaels, Yale S., Jamilly, Max, Ferry, Quentin R. V., Barbosa, Hector, Milne, Thomas A., Fulga, Tudor A.
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2019
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6445147/
https://ncbi.nlm.nih.gov/pubmed/30940799
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-019-09148-3
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