In situ functional dissection of RNA cis-regulatory elements by multiplex CRISPR-Cas9 genome engineering

RNA regulatory elements (RREs) are an important yet relatively under-explored facet of gene regulation. Deciphering the prevalence and functional impact of this post-transcriptional control layer requires technologies for disrupting RREs without perturbing cellular homeostasis. Here we describe geno...

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發表在:Nat Commun
Main Authors: Wu, Qianxin, Ferry, Quentin R. V., Baeumler, Toni A., Michaels, Yale S., Vitsios, Dimitrios M., Habib, Omer, Arnold, Roland, Jiang, Xiaowei, Maio, Stefano, Steinkraus, Bruno R., Tapia, Marta, Piazza, Paolo, Xu, Ni, Holländer, Georg A., Milne, Thomas A., Kim, Jin-Soo, Enright, Anton J., Bassett, Andrew R., Fulga, Tudor A.
格式: Artigo
語言:Inglês
出版: Nature Publishing Group UK 2017
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC5727397/
https://ncbi.nlm.nih.gov/pubmed/29235467
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-017-00686-2
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