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Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression
Spatial/temporal control of Cas9 guide RNA expression could considerably expand the utility of CRISPR-based technologies. Current approaches based on tRNA processing offer a promising strategy but suffer from high background. Here, to address this limitation, we present a screening platform which al...
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| Publicado no: | Nat Commun |
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| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group UK
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6445147/ https://ncbi.nlm.nih.gov/pubmed/30940799 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-019-09148-3 |
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