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Discovery and Characterization of a Thioesterase-Specific Monoclonal Antibody That Recognizes the 6-Deoxyerythronolide B Synthase

Assembly line polyketide synthases (PKSs) are large multimodular enzymes responsible for the biosynthesis of diverse antibiotics in bacteria. Structural and mechanistic analysis of these megasynthases can benefit from the discovery of reagents that recognize individual domains or linkers in a site-s...

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Détails bibliographiques
Publié dans:Biochemistry
Auteurs principaux: Li, Xiuyuan, Sevillano, Natalia, Greca, Florencia La, Hsu, Jake, Mathews, Irimpan I., Matsui, Tsutomu, Craik, Charles S., Khosla, Chaitan
Format: Artigo
Langue:Inglês
Publié: 2018
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6424575/
https://ncbi.nlm.nih.gov/pubmed/30289692
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.8b00886
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