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Structure of an Engineered Intein Reveals Thiazoline Ring and Provides Mechanistic Insight
We have engineered an intein which spontaneously and reversibly forms a thiazoline ring at the native N-terminal Lys-Cys splice junction. We identified conditions to stablize the thiazoline ring and provide the first crystallographic evidence, at 1.54 Å resolution, for its existence at an intein act...
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| Publicado no: | Biotechnol Bioeng |
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| Main Authors: | , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6399034/ https://ncbi.nlm.nih.gov/pubmed/30450538 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/bit.26875 |
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