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Backbone assignments of mini-RecA intein with short native exteins and an active N-terminal catalytic cysteine

The backbone resonance assignments of an engineered splicing-inactive mini-RecA intein based on triple resonance experiments with [(13)C,(15)N]-labeled protein are reported. The construct contains inactivating mutations specifically designed to retain most catalytic residues, especially those that a...

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Bibliografiska uppgifter
I publikationen:Biomol NMR Assign
Huvudupphovsmän: Pearson, C. Seth, Belfort, Georges, Belfort, Marlene, Shekhtman, Alexander
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2014
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC4385508/
https://ncbi.nlm.nih.gov/pubmed/25281002
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12104-014-9581-z
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