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Backbone Dynamics and Global Effects of an Activating Mutation in Minimized Mtu RecA Inteins

Inteins mediate protein splicing, which has found many applications in biotechnology and protein engineering. A single valine-to-leucine mutation (V67L) can globally enhance splicing and related cleavage reactions in minimized Mtu RecA inteins. However, the V67L mutation causes little change in crys...

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Detalhes bibliográficos
Main Authors: Du, Zhenming, Liu, Yangzhong, Ban, David, Lopez, Maria M., Belfort, Marlene, Wang, Chunyu
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2914542/
https://ncbi.nlm.nih.gov/pubmed/20562025
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2010.05.044
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