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Backbone Dynamics and Global Effects of an Activating Mutation in Minimized Mtu RecA Inteins
Inteins mediate protein splicing, which has found many applications in biotechnology and protein engineering. A single valine-to-leucine mutation (V67L) can globally enhance splicing and related cleavage reactions in minimized Mtu RecA inteins. However, the V67L mutation causes little change in crys...
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| Auteurs principaux: | , , , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2010
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2914542/ https://ncbi.nlm.nih.gov/pubmed/20562025 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2010.05.044 |
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