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Backbone assignments of mini-RecA intein with short native exteins and an active N-terminal catalytic cysteine
The backbone resonance assignments of an engineered splicing-inactive mini-RecA intein based on triple resonance experiments with [(13)C,(15)N]-labeled protein are reported. The construct contains inactivating mutations specifically designed to retain most catalytic residues, especially those that a...
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| Publicat a: | Biomol NMR Assign |
|---|---|
| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2014
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4385508/ https://ncbi.nlm.nih.gov/pubmed/25281002 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12104-014-9581-z |
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