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Structure of an Engineered Intein Reveals Thiazoline Ring and Provides Mechanistic Insight

We have engineered an intein which spontaneously and reversibly forms a thiazoline ring at the native N-terminal Lys-Cys splice junction. We identified conditions to stablize the thiazoline ring and provide the first crystallographic evidence, at 1.54 Å resolution, for its existence at an intein act...

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Bibliografski detalji
Izdano u:Biotechnol Bioeng
Glavni autori: Pearson, C. Seth, Nemati, Reza, Liu, Binbin, Zhang, Jing, Scalabrin, Matteo, Li, Zhong, Li, Hongmin, Fabris, Dan, Belfort, Marlene, Belfort, Georges
Format: Artigo
Jezik:Inglês
Izdano: 2019
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6399034/
https://ncbi.nlm.nih.gov/pubmed/30450538
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/bit.26875
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