Biophysical Characterization of a Disabled Double Mutant of Soybean Lipoxygenase: The “Undoing” of Precise Substrate Positioning Relative to Metal Cofactor and an Identified Dynamical Network

Soybean lipoxygenase (SLO) has served as a prototype for understanding the molecular origin of enzymatic rate accelerations. The double mutant (DM) L546A/L754A is considered a dramatic outlier, due to the unprecedented size and near temperature-independence of its primary kinetic isotope effect, low...

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Veröffentlicht in:J Am Chem Soc
Hauptverfasser: Hu, Shenshen, Offenbacher, Adam R., Thompson, Erin M., Gee, Christine L., Wilcoxen, Jarett, Carr, Cody A. M., Prigozhin, Daniil M., Yang, Vanessa, Alber, Tom, Britt, R. David, Fraser, James S., Klinman, Judith
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2019
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6353671/
https://ncbi.nlm.nih.gov/pubmed/30645119
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.8b10992
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