A new approach to Cas9-based genome editing in Aspergillus niger that is precise, efficient and selectable

Aspergillus niger and other filamentous fungi are widely used in industry, but efficient genetic engineering of these hosts remains nascent. For example, while molecular genetic tools have been developed, including CRISPR/Cas9, facile genome engineering of A. niger remains challenging. To address th...

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Detalles Bibliográficos
Publicado en:PLoS One
Main Authors: Leynaud-Kieffer, Laure M. C., Curran, Samuel C., Kim, Irene, Magnuson, Jon K., Gladden, John M., Baker, Scott E., Simmons, Blake A.
Formato: Artigo
Idioma:Inglês
Publicado: Public Library of Science 2019
Assuntos:
Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6336261/
https://ncbi.nlm.nih.gov/pubmed/30653574
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0210243
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