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Single-step Precision Genome Editing in Yeast Using CRISPR-Cas9

Genome modification in budding yeast has been extremely successful largely due to its highly efficient homology-directed DNA repair machinery. Several methods for modifying the yeast genome have previously been described, many of them involving at least two-steps: insertion of a selectable marker an...

תיאור מלא

שמור ב:
מידע ביבליוגרפי
הוצא לאור ב:Bio Protoc
Main Authors: Akhmetov, Azat, Laurent, Jon M, Gollihar, Jimmy, Gardner, Elizabeth C, Garge, Riddhiman K, Ellington, Andrew D, Kachroo, Aashiq H, Marcotte, Edward M
פורמט: Artigo
שפה:Inglês
יצא לאור: Bio-Protocol 2018
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC5951413/
https://ncbi.nlm.nih.gov/pubmed/29770349
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2765
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