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Single-step Precision Genome Editing in Yeast Using CRISPR-Cas9
Genome modification in budding yeast has been extremely successful largely due to its highly efficient homology-directed DNA repair machinery. Several methods for modifying the yeast genome have previously been described, many of them involving at least two-steps: insertion of a selectable marker an...
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| Publicado no: | Bio Protoc |
|---|---|
| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Bio-Protocol
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5951413/ https://ncbi.nlm.nih.gov/pubmed/29770349 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2765 |
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