Single-step Precision Genome Editing in Yeast Using CRISPR-Cas9

Genome modification in budding yeast has been extremely successful largely due to its highly efficient homology-directed DNA repair machinery. Several methods for modifying the yeast genome have previously been described, many of them involving at least two-steps: insertion of a selectable marker an...

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Bibliographische Detailangaben
Veröffentlicht in:Bio Protoc
Hauptverfasser: Akhmetov, Azat, Laurent, Jon M, Gollihar, Jimmy, Gardner, Elizabeth C, Garge, Riddhiman K, Ellington, Andrew D, Kachroo, Aashiq H, Marcotte, Edward M
Format: Artigo
Sprache:Inglês
Veröffentlicht: Bio-Protocol 2018
Schlagworte:
Methods Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5951413/
https://ncbi.nlm.nih.gov/pubmed/29770349
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2765
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