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Heterologous and endogenous U6 snRNA promoters enable CRISPR/Cas9 mediated genome editing in Aspergillus niger

BACKGROUND: U6 promoters have been used for single guide RNA (sgRNA) transcription in the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas9) genome editing system. However, no available U6 promoters have been identified in Aspergillus niger, which is an...

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Detalhes bibliográficos
Publicado no:Fungal Biol Biotechnol
Main Authors: Zheng, Xiaomei, Zheng, Ping, Sun, Jibin, Kun, Zhang, Ma, Yanhe
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2018
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5806293/
https://ncbi.nlm.nih.gov/pubmed/29456867
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s40694-018-0047-4
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