Harnessing heterologous and endogenous CRISPR-Cas machineries for efficient markerless genome editing in Clostridium

Application of CRISPR-Cas9 systems has revolutionized genome editing across all domains of life. Here we report implementation of the heterologous Type II CRISPR-Cas9 system in Clostridium pasteurianum for markerless genome editing. Since 74% of species harbor CRISPR-Cas loci in Clostridium, we also...

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Bibliografische gegevens
Gepubliceerd in:Sci Rep
Hoofdauteurs: Pyne, Michael E., Bruder, Mark R., Moo-Young, Murray, Chung, Duane A., Chou, C. Perry
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Nature Publishing Group 2016
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Article
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4860712/
https://ncbi.nlm.nih.gov/pubmed/27157668
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep25666
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