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Harnessing heterologous and endogenous CRISPR-Cas machineries for efficient markerless genome editing in Clostridium
Application of CRISPR-Cas9 systems has revolutionized genome editing across all domains of life. Here we report implementation of the heterologous Type II CRISPR-Cas9 system in Clostridium pasteurianum for markerless genome editing. Since 74% of species harbor CRISPR-Cas loci in Clostridium, we also...
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| Publicado no: | Sci Rep |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4860712/ https://ncbi.nlm.nih.gov/pubmed/27157668 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep25666 |
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