Engineering Plant Secondary Metabolism in Microbial Systems

Por Pyne, Michael E., Narcross, Lauren, Martin, Vincent J.J.
Publicado no Plant Physiol (2019)

Development of an electrotransformation protocol for genetic manipulation of Clostridium pasteurianum

Por Pyne, Michael E, Moo-Young, Murray, Chung, Duane A, Chou, C Perry
Publicado em 2013

Expansion of the genetic toolkit for metabolic engineering of Clostridium pasteurianum: chromosomal gene disruption of the endogenous CpaAI restriction enzyme

Por Pyne, Michael E, Moo-Young, Murray, Chung, Duane A, Chou, C Perry
Publicado no Biotechnol Biofuels (2014)

Coupling the CRISPR/Cas9 System with Lambda Red Recombineering Enables Simplified Chromosomal Gene Replacement in Escherichia coli

Por Pyne, Michael E., Moo-Young, Murray, Chung, Duane A., Chou, C. Perry
Publicado no Appl Environ Microbiol (2015)

Genome-directed analysis of prophage excision, host defence systems, and central fermentative metabolism in Clostridium pasteurianum

Por Pyne, Michael E., Liu, Xuejia, Moo-Young, Murray, Chung, Duane A., Chou, C. Perry
Publicado no Sci Rep (2016)

Harnessing heterologous and endogenous CRISPR-Cas machineries for efficient markerless genome editing in Clostridium

Por Pyne, Michael E., Bruder, Mark R., Moo-Young, Murray, Chung, Duane A., Chou, C. Perry
Publicado no Sci Rep (2016)

Extending CRISPR-Cas9 Technology from Genome Editing to Transcriptional Engineering in the Genus Clostridium

Por Bruder, Mark R., Pyne, Michael E., Moo-Young, Murray, Chung, Duane A., Chou, C. Perry
Publicado no Appl Environ Microbiol (2016)

Using the Endogenous CRISPR-Cas System of Heliobacterium modesticaldum To Delete the Photochemical Reaction Center Core Subunit Gene

Por Baker, Patricia L., Orf, Gregory S., Kevershan, Kimberly, Pyne, Michael E., Bicer, Taner, Redding, Kevin E.
Publicado no Appl Environ Microbiol (2019)

Improved Draft Genome Sequence of Clostridium pasteurianum Strain ATCC 6013 (DSM 525) Using a Hybrid Next-Generation Sequencing Approach

Por Pyne, Michael E., Utturkar, Sagar, Brown, Steven D., Moo-Young, Murray, Chung, Duane A., Chou, C. Perry
Publicado em 2014

An Engineered Aro1 Protein Degradation Approach for Increased cis,cis-Muconic Acid Biosynthesis in Saccharomyces cerevisiae

Por Pyne, Michael E., Narcross, Lauren, Melgar, Mindy, Kevvai, Kaspar, Mookerjee, Shoham, Leite, Gustavo B., Martin, Vincent J. J.
Publicado no Appl Environ Microbiol (2018)

A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids

Por Pyne, Michael E., Kevvai, Kaspar, Grewal, Parbir S., Narcross, Lauren, Choi, Brian, Bourgeois, Leanne, Dueber, John E., Martin, Vincent J. J.
Publicado no Nat Commun (2020)

Disruption of the Reductive 1,3-Propanediol Pathway Triggers Production of 1,2-Propanediol for Sustained Glycerol Fermentation by Clostridium pasteurianum

Por Pyne, Michael E., Sokolenko, Stanislav, Liu, Xuejia, Srirangan, Kajan, Bruder, Mark R., Aucoin, Marc G., Moo-Young, Murray, Chung, Duane A., Chou, C. Perry
Publicado no Appl Environ Microbiol (2016)