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Efficient genome editing using tRNA promoter-driven CRISPR/Cas9 gRNA in Aspergillus niger

As a powerful tool for fast and precise genome editing, the CRISPR/Cas9 system has been applied in filamentous fungi to improve the efficiency of genome alteration. However, the method of delivering guide RNA (gRNA) remains a bottleneck in performing CRISPR mutagenesis in Aspergillus species. Here w...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:PLoS One
Main Authors: Song, Letian, Ouedraogo, Jean-Paul, Kolbusz, Magdalena, Nguyen, Thi Truc Minh, Tsang, Adrian
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science 2018
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6108506/
https://ncbi.nlm.nih.gov/pubmed/30142205
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0202868
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