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Tools and protocol for quantification of myosin phosphorylation with MRM-MS

The phosphorylation of myosin regulatory light chain (LC20) at Thr18 and Ser19 is positively correlated with tension development in smooth muscle tissue, and the molar stoichiometry of LC20 phosphorylation is commonly profiled as a measure of smooth muscle contractility. We provide details for a new...

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Vydáno v:MethodsX
Hlavní autoři: MacDonald, Justin A., Ulke-Lemée, Annegret, Chappellaz, Mona, Segboer, Hayden
Médium: Artigo
Jazyk:Inglês
Vydáno: Elsevier 2018
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6314968/
https://ncbi.nlm.nih.gov/pubmed/30622916
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2018.05.004
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