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Tools and protocol for quantification of myosin phosphorylation with MRM-MS

The phosphorylation of myosin regulatory light chain (LC20) at Thr18 and Ser19 is positively correlated with tension development in smooth muscle tissue, and the molar stoichiometry of LC20 phosphorylation is commonly profiled as a measure of smooth muscle contractility. We provide details for a new...

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Detalhes bibliográficos
Publicado no:MethodsX
Main Authors: MacDonald, Justin A., Ulke-Lemée, Annegret, Chappellaz, Mona, Segboer, Hayden
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6314968/
https://ncbi.nlm.nih.gov/pubmed/30622916
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2018.05.004
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