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Tools and protocol for quantification of myosin phosphorylation with MRM-MS

The phosphorylation of myosin regulatory light chain (LC20) at Thr18 and Ser19 is positively correlated with tension development in smooth muscle tissue, and the molar stoichiometry of LC20 phosphorylation is commonly profiled as a measure of smooth muscle contractility. We provide details for a new...

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Veröffentlicht in:MethodsX
Hauptverfasser: MacDonald, Justin A., Ulke-Lemée, Annegret, Chappellaz, Mona, Segboer, Hayden
Format: Artigo
Sprache:Inglês
Veröffentlicht: Elsevier 2018
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6314968/
https://ncbi.nlm.nih.gov/pubmed/30622916
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2018.05.004
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