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A Rapid Extraction Method for mammalian cell cultures, suitable for quantitative immunoblotting analysis of proteins, including phosphorylated GCN2 and eIF2α

Many studies require the detection and relative quantitation of proteins from cell culture samples using immunoblotting. Limiting factors are the cost of protease inhibitors, the time required to break cells and generate samples, as well as the high risk of protein loss during cell breakage procedur...

Πλήρης περιγραφή

Αποθηκεύτηκε σε:
Λεπτομέρειες βιβλιογραφικής εγγραφής
Τόπος έκδοσης:MethodsX
Κύριοι συγγραφείς: Silva, Richard C., Castilho, Beatriz A., Sattlegger, Evelyn
Μορφή: Artigo
Γλώσσα:Inglês
Έκδοση: Elsevier 2017
Θέματα:
Διαθέσιμο Online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6314271/
https://ncbi.nlm.nih.gov/pubmed/30619721
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2017.10.008
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