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Accurate quantitation of phospholamban phosphorylation by immunoblot

We have developed a quantitative immunoblot method to measure the mole fraction of phospholamban (PLB) phosphorylated at Ser16 (X(p)) in biological samples. In cardiomyocytes, PLB phosphorylation activates the sarcoplasmic reticulum calcium ATPase (SERCA), which reduces cytoplasmic Ca(++) to relax t...

詳細記述

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書誌詳細
主要な著者: Ablorh, Naa-Adjeley, Miller, Tyler, Nitu, Florentin, Gruber, Simon J., Karim, Christine, Thomas, David D.
フォーマット: Artigo
言語:Inglês
出版事項: 2012
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC3338889/
https://ncbi.nlm.nih.gov/pubmed/22369895
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2012.01.028
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