Accurate quantitation of phospholamban phosphorylation by immunoblot

We have developed a quantitative immunoblot method to measure the mole fraction of phospholamban (PLB) phosphorylated at Ser16 (X(p)) in biological samples. In cardiomyocytes, PLB phosphorylation activates the sarcoplasmic reticulum calcium ATPase (SERCA), which reduces cytoplasmic Ca(++) to relax t...

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Bibliographische Detailangaben
Hauptverfasser: Ablorh, Naa-Adjeley, Miller, Tyler, Nitu, Florentin, Gruber, Simon J., Karim, Christine, Thomas, David D.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2012
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3338889/
https://ncbi.nlm.nih.gov/pubmed/22369895
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2012.01.028
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