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Accurate quantitation of phospholamban phosphorylation by immunoblot
We have developed a quantitative immunoblot method to measure the mole fraction of phospholamban (PLB) phosphorylated at Ser16 (X(p)) in biological samples. In cardiomyocytes, PLB phosphorylation activates the sarcoplasmic reticulum calcium ATPase (SERCA), which reduces cytoplasmic Ca(++) to relax t...
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| Главные авторы: | , , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2012
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3338889/ https://ncbi.nlm.nih.gov/pubmed/22369895 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2012.01.028 |
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