Active site residue identity regulates cleavage preference of LAGLIDADG homing endonucleases

LAGLIDADG homing endonucleases (meganucleases) are site-specific mobile endonucleases that can be adapted for genome-editing applications. However, one problem when reprogramming meganucleases on non-native substrates is indirect readout of DNA shape and flexibility at the central 4 bases where clea...

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Bibliographic Details
Published in:Nucleic Acids Res
Main Authors: McMurrough, Thomas A, Brown, Christopher M, Zhang, Kun, Hausner, Georg, Junop, Murray S, Gloor, Gregory B, Edgell, David R
Format: Artigo
Language:Inglês
Published: Oxford University Press 2018
Subjects:
Nucleic Acid Enzymes
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6294521/
https://ncbi.nlm.nih.gov/pubmed/30357419
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gky976
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