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Synthesis of Fluorescence Turn-On DNA Hybridization Probe using the (DEA)tC 2’-deoxycytidine Analogue

(DEA)tC is a tricyclic 2’-deoxycytidine analogue that can be incorporated into oligonucleotides by solid-phase synthesis and that exhibits a large fluorescence enhancement when correctly base-paired with guanine base in a DNA–DNA duplex. The synthesis of (DEA)tC begins with 5-amino-2-methylbenzothia...

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Detalhes bibliográficos
Publicado no:Curr Protoc Nucleic Acid Chem
Main Authors: Turner, M. Benjamin, Anderson, Brooke A., Samaan, George N., Coste, Michael, Burns, Dillon D., Purse, Byron W.
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6284819/
https://ncbi.nlm.nih.gov/pubmed/30369083
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpnc.59
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