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Superior protein titers in half the fermentation time: Promoter and process engineering for the glucose‐regulated GTH1 promoter of Pichia pastoris

Protein production in Pichia pastoris is often based on the methanol‐inducible P (AOX1) promoter which drives the expression of the target gene. The use of methanol has major drawbacks, so there is a demand for alternative promoters with good induction properties such as the glucose‐regulated P (GTH...

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Detalhes bibliográficos
Publicado no:Biotechnol Bioeng
Main Authors: Prielhofer, Roland, Reichinger, Michaela, Wagner, Nina, Claes, Katrien, Kiziak, Christoph, Gasser, Brigitte, Mattanovich, Diethard
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6221138/
https://ncbi.nlm.nih.gov/pubmed/30016537
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/bit.26800
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