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CRISPR/Cas9-mediated gene targeting during embryogenesis in swine

Ability to disrupt genes is essential in elucidating gene function. Unlike rodents or amphibians, it has been difficult to generate gene targeted embryos in large animals. Therefore, studies of early embryo development have been hampered due to the limitation. A recent technology suggests that targe...

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Bibliographic Details
Published in:Methods Mol Biol
Main Authors: Ryu, Junghyun, Lee, Kiho
Format: Artigo
Language:Inglês
Published: 2017
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6205924/
https://ncbi.nlm.nih.gov/pubmed/28456969
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-6988-3_16
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