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BtsCI and BseGI display sequence preference in the nucleotides flanking the recognition sequence

Restriction enzymes are the bread and butter of Molecular Biology. Nonetheless, how restriction enzymes recognize and cleave their target is not always clear. When developing a method for the enzymatic production of oligonucleotides, we noticed that type II endonucleases BtsCI and BseGI, which recog...

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Pubblicato in:PLoS One
Autori principali: Rosa, João, Fernandez-Gonzalez, Esther, Ducani, Cosimo, Högberg, Björn
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science 2018
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6097692/
https://ncbi.nlm.nih.gov/pubmed/30118487
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0202057
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