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BtsCI and BseGI display sequence preference in the nucleotides flanking the recognition sequence

Restriction enzymes are the bread and butter of Molecular Biology. Nonetheless, how restriction enzymes recognize and cleave their target is not always clear. When developing a method for the enzymatic production of oligonucleotides, we noticed that type II endonucleases BtsCI and BseGI, which recog...

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Bibliografiske detaljer
Udgivet i:PLoS One
Main Authors: Rosa, João, Fernandez-Gonzalez, Esther, Ducani, Cosimo, Högberg, Björn
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2018
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6097692/
https://ncbi.nlm.nih.gov/pubmed/30118487
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0202057
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