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Engineering Nt.BtsCI and Nb.BtsCI nicking enzymes and applications in generating long overhangs
Type IIS restriction endonuclease BtsCI (GGATG 2/0) is a neoschizomer of FokI (GGATG 9/13) and cleaves closer to the recognition sequence. Although M.BtsCI shows 62% amino acid sequence identity to M.FokI, BtsCI and FokI restriction endonucleases do not share significant amino acid sequence similari...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2831314/ https://ncbi.nlm.nih.gov/pubmed/19955230 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp1092 |
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