Engineering Nt.BtsCI and Nb.BtsCI nicking enzymes and applications in generating long overhangs

Type IIS restriction endonuclease BtsCI (GGATG 2/0) is a neoschizomer of FokI (GGATG 9/13) and cleaves closer to the recognition sequence. Although M.BtsCI shows 62% amino acid sequence identity to M.FokI, BtsCI and FokI restriction endonucleases do not share significant amino acid sequence similari...

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Bibliographic Details
Main Authors: Too, Priscilla Hiu-Mei, Zhu, Zhenyu, Chan, Siu-Hong, Xu, Shuang-yong
Format: Artigo
Language:Inglês
Published: Oxford University Press 2010
Subjects:
Nucleic Acid Enzymes
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2831314/
https://ncbi.nlm.nih.gov/pubmed/19955230
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp1092
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