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BtsCI and BseGI display sequence preference in the nucleotides flanking the recognition sequence

Restriction enzymes are the bread and butter of Molecular Biology. Nonetheless, how restriction enzymes recognize and cleave their target is not always clear. When developing a method for the enzymatic production of oligonucleotides, we noticed that type II endonucleases BtsCI and BseGI, which recog...

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Foilsithe in:PLoS One
Main Authors: Rosa, João, Fernandez-Gonzalez, Esther, Ducani, Cosimo, Högberg, Björn
Formáid: Artigo
Teanga:Inglês
Foilsithe: Public Library of Science 2018
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6097692/
https://ncbi.nlm.nih.gov/pubmed/30118487
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0202057
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