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BtsCI and BseGI display sequence preference in the nucleotides flanking the recognition sequence

Restriction enzymes are the bread and butter of Molecular Biology. Nonetheless, how restriction enzymes recognize and cleave their target is not always clear. When developing a method for the enzymatic production of oligonucleotides, we noticed that type II endonucleases BtsCI and BseGI, which recog...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:PLoS One
Main Authors: Rosa, João, Fernandez-Gonzalez, Esther, Ducani, Cosimo, Högberg, Björn
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science 2018
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6097692/
https://ncbi.nlm.nih.gov/pubmed/30118487
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0202057
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