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Newly revised protocol for quantitative PCR-based assay to measure mitochondrial and nuclear DNA damage

Given the crucial role of DNA damage in human health and disease, it is important to be able to accurately measure both mitochondrial (mtDNA) and nuclear (nDNA) damage. In this unit, we describe a method based on a long-amplicon quantitative PCR-based assay; the main strength of the assay is that a...

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Vydáno v:Curr Protoc Toxicol
Hlavní autoři: Sanders, Laurie H., Rouanet, Jeremy P., Howlett, Evan H., Leuthner, Tess C., Rooney, John P., Greenamyre, J. Timothy, Meyer, Joel N.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2018
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6060631/
https://ncbi.nlm.nih.gov/pubmed/30040241
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cptx.50
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