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PCR Based Determination of Mitochondrial DNA Copy Number in Multiple Species

Mitochondrial DNA (mtDNA) copy number is a critical component of overall mitochondrial health. In this chapter we describe methods for isolation of both mtDNA and nuclear DNA (nucDNA), and measurement of their respective copy numbers using quantitative PCR. Methods differ depending on the species an...

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Detalhes bibliográficos
Publicado no:Methods Mol Biol
Main Authors: Rooney, JP, Ryde, IT, Sanders, LH, Howlett, EH, Colton, MD, Germ, KE, Mayer, GD, Greenamyre, JT, Meyer, JN
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4312664/
https://ncbi.nlm.nih.gov/pubmed/25308485
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-1875-1_3
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