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Quantitative PCR-Based Measurement of Nuclear and Mitochondrial DNA Damage and Repair in Mammalian Cells

In this chapter, we describe a gene-specific quantitative PCR (QPCR)-based assay for the measurement of DNA damage, using amplification of long DNA targets. This assay has been used extensively to measure the integrity of both nuclear and mitochondrial genomes exposed to different genotoxins and has...

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Vydáno v:Methods Mol Biol
Hlavní autoři: Furda, Amy, Santos, Janine H., Meyer, Joel N., Van Houten, Bennett
Médium: Artigo
Jazyk:Inglês
Vydáno: 2014
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4407362/
https://ncbi.nlm.nih.gov/pubmed/24623245
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-739-6_31
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