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Highly active enzymes by automated combinatorial backbone assembly and sequence design

Automated design of enzymes with wild-type-like catalytic properties has been a long-standing but elusive goal. Here, we present a general, automated method for enzyme design through combinatorial backbone assembly. Starting from a set of homologous yet structurally diverse enzyme structures, the me...

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Detalhes bibliográficos
Publicado no:Nat Commun
Main Authors: Lapidoth, Gideon, Khersonsky, Olga, Lipsh, Rosalie, Dym, Orly, Albeck, Shira, Rogotner, Shelly, Fleishman, Sarel J.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6050298/
https://ncbi.nlm.nih.gov/pubmed/30018322
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-018-05205-5
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