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Pathways of Metabolite-related Damage to A Synthetic p53 Gene Exon 7 Oligonucleotide using Magnetic Enzyme Bioreactor Beads and LC-MS/MS Sequencing

Reactive metabolites of environmental chemicals and drugs can cause site-specific damage to p53 tumor suppressor gene in a major pathway for genotoxicity. We report here a high throughput, cell-free, 96-well plate magnetic bead-enzyme system interfaced with LCMS/MS sequencing to bioactivate test che...

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Detalhes bibliográficos
Publicado no:Biochemistry
Main Authors: Malla, Spundana, Kadimisetty, Karteek, Jiang, Di, Choudhary, Dharamainder, Rusling, James F.
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6030469/
https://ncbi.nlm.nih.gov/pubmed/29750510
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.8b00271
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