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Characterization of trimethoprim resistant E. coli dihydrofolate reductase mutants by mass spectrometry and inhibition by propargyl-linked antifolates

Pathogenic Escherichia coli, one of the primary causes of urinary tract infections, has shown significant resistance to the most popular antibiotic, trimethoprim (TMP), which inhibits dihydrofolate reductase (DHFR). The resistance is modulated by single point mutations of DHFR. The impact of two cli...

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Veröffentlicht in:Chem Sci
Hauptverfasser: Cammarata, Michael, Thyer, Ross, Lombardo, Michael, Anderson, Amy, Wright, Dennis, Ellington, Andrew, Brodbelt, Jennifer S.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Royal Society of Chemistry 2017
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6020862/
https://ncbi.nlm.nih.gov/pubmed/29967675
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c6sc05235e
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