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Evaluation of bisulfite kits for DNA methylation profiling in terms of DNA fragmentation and DNA recovery using digital PCR

DNA methylation is one of the most important epigenetic modifications in the regulation of gene transcription. The current gold standard to study this modification is bisulfite sequencing. Although multiple commercial bisulfite treatment kits provide good conversion efficiencies, DNA loss and especi...

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Podrobná bibliografie
Vydáno v:PLoS One
Hlavní autoři: Kint, Sam, De Spiegelaere, Ward, De Kesel, Jonas, Vandekerckhove, Linos, Van Criekinge, Wim
Médium: Artigo
Jazyk:Inglês
Vydáno: Public Library of Science 2018
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6002050/
https://ncbi.nlm.nih.gov/pubmed/29902267
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0199091
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