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Evaluation of bisulfite kits for DNA methylation profiling in terms of DNA fragmentation and DNA recovery using digital PCR

DNA methylation is one of the most important epigenetic modifications in the regulation of gene transcription. The current gold standard to study this modification is bisulfite sequencing. Although multiple commercial bisulfite treatment kits provide good conversion efficiencies, DNA loss and especi...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Kint, Sam, De Spiegelaere, Ward, De Kesel, Jonas, Vandekerckhove, Linos, Van Criekinge, Wim
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6002050/
https://ncbi.nlm.nih.gov/pubmed/29902267
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0199091
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